A simple screening procedure for microbial phase-transfer mediators conveying anions
✍ Scribed by C. Stengel; G. Reinhardt; Dr. U. Gräfe
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 446 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The conveyance of methylorange (helianthin) anion from a weakly acidic aqueous solution to a toluene layer was taken as a measure for the presence of anionophoric metabolites in microbial culture extracts mediating the transfer of amphiphilic anions from the water phase to an organic solvent. The presence of lipophilic amines such as pamamycin in microbial extracts thus could easily be detected by a directed screening procedure for anionophoric compounds. The efficiency of the screening procedure was demonstrated both with purified antiobiotics (pamamycin, a dimethylamino subunit of pamamycin, turimycin) and extracts from a large collection of Streptomyces strains.