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A simple and inexpensive procedure for preparative polyacrylamide gel electrophoresis of RNA

✍ Scribed by Allan Jacobson; Harvey F. Lodish

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
249 KB
Volume
54
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method

that is both simple and inexpensive is described for preparative polyacrylamide gel electrophoresis of low-molecular-weight RNA. The utility of the method is demonstrated by the preparative separation of 4S and 5s RX-i.

πŸ“œ SIMILAR VOLUMES

A convenient, efficient, and inexpensive
A convenient, efficient, and inexpensive method for scintillation counting of polyacrylamide gel slices after electrophoresis
✍ GΓΆran Gezelius πŸ“‚ Article πŸ“… 1977 πŸ› Elsevier Science 🌐 English βš– 422 KB

A method is described for analyzing the radioactivity of 3H-labeled RNA after separation by gel electrophoresis. The gels were soaked in 10% acetic acid and were sliced. The gel slices were dehydrated in alcohol, then saturated with toluene, and finally permeated with a toluene-based scintillation f