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A convenient, efficient, and inexpensive method for scintillation counting of polyacrylamide gel slices after electrophoresis

✍ Scribed by Göran Gezelius

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
422 KB
Volume
80
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for analyzing the radioactivity of 3H-labeled RNA after separation by gel electrophoresis. The gels were soaked in 10% acetic acid and were sliced. The gel slices were dehydrated in alcohol, then saturated with toluene, and finally permeated with a toluene-based scintillation fluid containing butyl-PBD. The radioactivity of RNA was then analyzed in sitrr in the gel slices using a liquid scintillator. The counting efficiency of this technique is high, about 58%. This is even slightly better than the counting efficiency attained after solubilization of the RNA in Soluene 350 (about 55%). With the same fluor, Permablend III. the counting efficiencies of the two techniques are alike.

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