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A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis

✍ Scribed by Gregory J. Wadsworth; Margaret G. Redinbaugh; John G. Scandalios

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
627 KB
Volume
172
Category
Article
ISSN
0003-2697

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✦ Synopsis

A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.

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