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Normalizations of RNA dot blots with oligo(dT)

โœ Scribed by Calvin B. Harley


Book ID
113288607
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
125 KB
Volume
4
Category
Article
ISSN
0168-9525

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Isolation of messenger RNA from polysome
โœ R.E. Pemberton; P. Liberti; C. Baglioni ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 665 KB

A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro