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Isolation of poly (A)RNA on oligo)dT)-cellulose columns

Book ID
113288312
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
65 KB
Volume
1
Category
Article
ISSN
0168-9525

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A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro

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