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Effect of flow rate on the isolation of polyadenylated RNA on oligo(dT)-cellulose colums

✍ Scribed by S. Nadin-Davis; V.A. Mezl

Book ID
118387595
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
234 KB
Volume
11
Category
Article
ISSN
0165-022X

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Isolation of messenger RNA from polysome
Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose
✍ R.E. Pemberton; P. Liberti; C. Baglioni πŸ“‚ Article πŸ“… 1975 πŸ› Elsevier Science 🌐 English βš– 665 KB

A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro