✦ LIBER ✦

Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA

✍ Scribed by John A. Bantle; Ian H. Maxwell; William E. Hahn

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 966 KB
Volume: 72
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90549-2

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Isolation of messenger RNA from polysome

Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose

✍ R.E. Pemberton; P. Liberti; C. Baglioni 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 665 KB

A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro

Isolation of nascent RNA molecules from

Isolation of nascent RNA molecules from rat liver nuclei: The use of mercurated CTP and the specificity of thiopropyl Sepharose chromatography

✍ Trevor J.C. Beebee; Denrick S. Carty 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 584 KB

Selective enhancement of tubulingene exp

Selective enhancement of tubulingene expression and increase in oligo (dT)-bound RNA in the rat brain after nigrostriatal pathway unilateral lesion and treatment with ganglioside

✍ E. Yavin; S. Gil; A. Consolazione; R. dal Toso; A. Leon 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 666 KB

Partial hemitransection of the rat nigrostriatal pathway has been applied to study changes in the expression of tubulin and actin cytoskeletal genes. RNA was isolated from ipsilateral and contralateral structures of substantia nigra and striatum tissues of lesioned or sham-operated animals by a comb

Sources of error in the determination of

Sources of error in the determination of specific radioactivity of amino acids isolated by ion-exchange chromatography

✍ M.K. Gaitonde; R.W.K. Nixey 📂 Article 📅 1972 🏛 Elsevier Science 🌐 English ⚖ 861 KB

The use of high-performance liquid chrom

The use of high-performance liquid chromatography in the isolation and analysis of oligoribonucleotides synthesized by the T4 RNA ligase reaction

✍ Larry W. McLaughlin; Elena Romaniuk 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 664 KB

High-performance liquid chromatography (HPLC) with both amino-propyl-silyl ion-exchange and octadecasilyl reverse-phase columns has been used to simultaneously analyze all substrates, intermediates, and products involved in the T4 RNA ligase reaction. Both singlestep ligations (e.g., UpApA + pCp) as

Refinements of the Differential Display

Refinements of the Differential Display Reverse Transcription–Polymerase Chain Reaction Technique: Use of Oligo(dT)-Based Anchored Primers with Escherichia coli Messenger RNA Identifies a Salt-Induced Promoter in the dcw Gene Cluster

✍ Rama Rao Amara; Vijaya Satchidanandam 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 100 KB

Differential display reverse transcription PCR (DDRT-PCR) 2 is a popular technique used to identify differences in messenger RNA species expressed under different conditions (1-4). The use of this technique in prokaryotic systems has been greatly hampered by the lack of a poly(A) tail at the 3Ј end