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Hybridization of Oligo(dT) to RNA on nitrocellulose

โœ Scribed by Harley, Calvin B.


Book ID
120063581
Publisher
Elsevier Science
Year
1987
Weight
469 KB
Volume
4
Category
Article
ISSN
0735-0651

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Isolation of messenger RNA from polysome
โœ R.E. Pemberton; P. Liberti; C. Baglioni ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 665 KB

A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro