Many mutant strains devoid of aminopeptidase activity have been isolated in Escherichia coli. All of them produce cross-reacting material when tested against specific antiaminopeptidase antibody. The map position of the locus specifying this enzyme has been determined by three conjugations and two P
Nitrofuratoin-resistant mutants of Escherichia coli: Isolation and mapping
β Scribed by Sastry, Srinivas S. ;Jayaraman, R.
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 221 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
Mutants of Escherichia coli resistant to nitrofurantoin have been isolated. The mutations, designated nfnA and nfnB were introduced individually into a multiply auxotrophic E. coli F- strain and mapped by conjugation and transduction. nfnA is located at 79.8 min and nfnB at 13.0 min on the E. coli chromosome.
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When arsenate-resistant mutants are selected approximately 50 per cent of them are also consitutive for the synthesis of alkaline phosphatase and the Pi-binding protein. Some of these mutants are linked to ilv (phoS- or phoT-), other are linked to proC (phoR-). One of the mutant strains linked to il