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Isolation and genetic mapping of Escherichia coli aminopeptidase mutants

✍ Scribed by Latil, Michèle ;Murgier, Maryse ;Lazdunski, Andrée ;Lazdunski, Claude

Publisher
Springer
Year
1976
Tongue
English
Weight
390 KB
Volume
148
Category
Article
ISSN
0026-8925

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✦ Synopsis

Many mutant strains devoid of aminopeptidase activity have been isolated in Escherichia coli. All of them produce cross-reacting material when tested against specific antiaminopeptidase antibody. The map position of the locus specifying this enzyme has been determined by three conjugations and two P1 mediated transduction experiments. By analogy with Salmonella typhimurium this locus has been called pepN (Miller, 1975). Mutations in pepN are jointly transduced with fabA and pyrD at high frequency. These data and conjugation results suggest a location between 20.5 and 22.5 minutes on E. coli genetic map.

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