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Nitration Mediated Activation of Akt Involved in Endothelial Nitric Oxide Synthase (eNOS) Mitochondria Translocation

✍ Scribed by Ruslan Rafikov; Saurabh Aggarwal; Olga Rafikova; Xutong Sun; Christine Gross; Yali Hou; Stephen M Black

Book ID
113632378
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
58 KB
Volume
51
Category
Article
ISSN
0891-5849

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med