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Estrogen induced changes in Akt-dependent activation of endothelial nitric oxide synthase and vasodilation

โœ Scribed by Maria Florian; Yan Lu; Mark Angle; Sheldon Magder


Book ID
116891715
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
265 KB
Volume
69
Category
Article
ISSN
0039-128X

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med