Next Generation Sequencing Advances, Applications and Challenges

✍ Scribed by Jerzy K Kulski

• Publisher: InTech
• Year: 2016
• Tongue: English
• Leaves: 449
• Category: Library

✦ Synopsis

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

✦ Table of Contents

Chapter 1   Next-Generation Sequencing — An Overview of the History, Tools, and “Omic” Applications by Jerzy K. Kulski
Chapter 2   Next Generation Sequencing in Aquatic Models by Yuan Lu, Yingjia Shen, Wesley Warren and Ronald Walter
Chapter 3   MHC Genotyping in Human and Nonhuman Species by PCRbased Next-Generation Sequencing by Takashi Shiina, Shingo Suzuki and Jerzy K. Kulski
Chapter 4   Transcriptomic Profiling Using Next Generation Sequencing - Advances, Advantages, and Challenges by Krishanpal Anamika, Srikant Verma, Abhay Jere and Aarti Desai
Chapter 5   Computational Analysis and Integration of MeDIP-seq Methylome Data by Gareth A. Wilson and Stephan Beck
Chapter 6   Analysis of Next-generation Sequencing Data in Virology - Opportunities and Challenges by Sunitha M. Kasibhatla, Vaishali P. Waman, Mohan M. Kale and Urmila Kulkarni-Kale
Chapter 7   RNA-seq – Revealing Biological Insights in Bacteria by Mariana P. Santana, Flavia F. Aburjaile, Mariana T.D. Parise, Sandeep Tiwari, Artur Silva, Vasco Azevedo and Anne Cybele Pinto
Chapter 8   Dealing with the Data Deluge – New Strategies in Prokaryotic Genome Analysis by Leonid Zaslavsky, Stacy Ciufo, Boris Fedorov, Boris Kiryutin, Igor Tolstoy and Tatiana Tatusova
Chapter 9   Reaping the Benefits of Next-generation Sequencing Technologies for Crop Improvement — Solanaceae by Sushil Satish Chhapekar, Rashmi Gaur, Ajay Kumar and Nirala Ramchiary
Chapter 10   Perspectives on the Application of Next-generation Sequencing to the Improvement of Africa’s Staple Food Crops by Melaku Gedil, Morag Ferguson, Gezahegn Girma, Andreas Gisel, Livia Stavolone and Ismail Rabbi
Chapter 11   Hop-variety Identification Using First- and Second-generation Sequencing by Hiromasa Yamauchi
Chapter 12   Analysis of Haplotype Sequences by Sally S. Lloyd, Edward J. Steele and Roger L. Dawkins
Chapter 13   On Genotyping Polymorphic HLA Genes — Ambiguities and Quality Measures Using NGS by Szilveszter Juhos, Krisztina Rigó and György Horváth
Chapter 14   DNA-based Diagnosis of Uncharacterized Inherited Macrothrombocytopenias Using Next-generation Sequencing Technology with a Candidate Gene Array by David J. Rabbolini, Marie-Christine Morel Kopp, Sara Gabrielli, Qiang Chen, William S. Stevenson and Christopher M. Ward
Chapter 15   Clinical Implementation of Next-generation Sequencing in the Field of Prenatal Diagnostics by Gwendolin Manegold-Brauer and Olav Lapaire
Chapter 16   Impact of Gene Annotation on RNA-seq Data Analysis by Shanrong Zhao and Baohong Zhang