Next Generation Sequencing Technologies and Challenges in Sequence Assembly

✍ Scribed by Sara El-Metwally, Osama M. Ouda, Mohamed Helmy (auth.)

Publisher: Springer-Verlag New York
Year: 2014
Tongue: English
Leaves: 123
Series: SpringerBriefs in Systems Biology 7
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field.

This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

✦ Table of Contents

Front Matter....Pages i-xii
Front Matter....Pages 1-1
Basics of Molecular Biology for Next-Generation Sequencing....Pages 3-13
Algorithms and Data Structures in Next-Generation Sequencing....Pages 15-26
Front Matter....Pages 27-27
First- and Next-Generations Sequencing Methods....Pages 29-36
Next-Generation Sequencing Platforms....Pages 37-44
Challenges in the Next-Generation Sequencing Field....Pages 45-49
New Horizons in Next-Generation Sequencing....Pages 51-59
Novel Next-Generation Sequencing Applications....Pages 61-70
Front Matter....Pages 71-71
Next-Generation Sequence Assembly Overview....Pages 73-78
Approaches and Challenges of Next-Generation Sequence Assembly Stages....Pages 79-93
Assessment of Next-Generation Sequence Assembly....Pages 95-101
Next-Generation Sequence Assemblers....Pages 103-116
Back Matter....Pages 117-118

✦ Subjects

Systems Biology; Human Genetics; Bioinformatics

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