New HPLC–MS method for quantitative determination of apovincaminic acid in human plasma

✍ Scribed by Laurian Vlase; Silvia Imre; Sorin Leucuta

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 639 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500284

✦ Synopsis

New HPLC-MS method for quantitative determination of apovincaminic acid in human plasma

A fast and sensitive HPLC -MS method for the quantification of apovincaminic acid, a vinpocetine metabolite, in human plasma has been developed and validated. After protein precipitation with methanol, 10 mL of supernatant was injected at 458C onto a Zorbax SB-C18 column. Elution was performed in less than three minutes with water containing 0.2% formic acid and acetonitrile (80:20) at 0.75 mL/min. A linearity domain between 4 and 240 ng/mL and a limit of quantification of 4 ng/mL apovincaminic acid were established by monitoring the signal corresponding to m/z = 323. Accuracy and precision were less than 5.2% for within-run assay and 10% for between-run assay. The method was successfully applied in a bioequivalence study of two pharmaceutical products containing 5 mg vinpocetine.