GLC method for quantitative determination of amitriptyline in human plasma

✍ Scribed by Howard B. Hucker; Susan C. Stauffer

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 216 KB
• Volume: 63
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600630228

✦ Synopsis

A GLC method was developed for the measurement of steady-state levels of amitriptyline and nortriptyline in human plasma after therapeutic doses of amitriptyline. The drugs were extracted from alkalinized samples with heptane-3% isoamyl alcohol and back-extracted into 0.1 N HCI. After subsequent extraction into ether, the drugs were analyzed by GLC. The method is accurate, specific, and sensitive, being capable of measuring concentrations as low .as 25 ng/ml of these drugs. After 6 days of amitriptyline administration (75 mg/day), levels of amitriptyline and nortriptyline in three subjects were approximately 40-90 and 40 ng/ml, respectively, 12 hr after the last dose.

Keyphrases

Amitriptyline-GLC determination in plasma 0 Nortriptyline-GLC determination in plasma 0 GLC-determination of steady-state levels of amitriptyline and nortriptyline in human plasma