## Abstract Two complimentary syntheses of selectively ^15^N‐ or ^13^C‐labelled malachite green hydrochloride were developed in order to provide labelled ligands for structural studies of RNA aptamer/ligand complexes. The ^15^N‐ and ^13^C‐labelled versions of the dye have been used in NMR studies t
New chiral synthons of 13C- or 15N-labelled α-amino acids
✍ Scribed by Alexander Popkov; Milan Nádvorník; Vratislav Langer; Robert Jirásko; Michal Holčapek; Tomáš Weidlich; Antonín Lyčka
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- French
- Weight
- 74 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract We have developed a stereospecific biosynthesis of ^13^C‐ and ^15^N‐labeled L‐serine which involves the serine‐type methylotroph __Methylobacterium extorquens__ AM1. In this biosynthesis, C‐3 of serine is derived from methanol while C‐2, C‐1 and the α‐amino group are derived from glycin
## Abstract Two types of multi‐labelled urocanic acids with stable isotopes, __i.e.__, [3‐^2^H,1′,3′‐^15^N~2~]urocanic acid and [2,3,5′‐^2^H~3~,2′‐^13^C,1′,3′‐^15^N~2~]urocanic acid were synthesized by the enzymatic reaction of DL‐[3,3‐^2^H~2~,1′,3′‐^15^N~2~]histidine or DL‐[2,3,3,5′‐^2^H~4~,2′‐^13
## Abstract We have developed a stereospecific chemomicrobiological synthesis of labeled tryptophan. L‐[3‐^13^C]Serine, [1‐^15^N]‐ and [2‐^13^C]indole were used as precursors for the synthesis of L‐[β‐^13^C]‐, L‐[1′‐^15^N]‐, and L‐[2′‐^13^C]tryptophan, respectively. The labeled precursors were inco
The vicinal "G'H, "N-'H and %-"N spin coupling constants were obtained from the 'H and =C NMR spectra of various ionic forms of the amino acids phenylalanine, a-aminobutyric acid and p-alanine, and also from their "N isotopomers. It is concluded that the "C-'H and =N-lH coupling constants are reliab
## Abstract Synthetic schemes for 2(S,R)‐amino‐3‐[D~3~](R,S)methylpentanoic acid, 2(S,R)‐amino‐3‐[^13^C](R,S)methyl‐[4‐^13^C]pentanoic acid (isoleucine) and [1‐^13^C,^15^N](S)‐leucine are described. The side chain labeled isoleucines were constructed by first alkylating 2,4,4‐trimethyl‐2‐oxazoline