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Stereoselective synthesis of stable isotope-labeled L-α-amino acids: Biosynthesis of 2H-, 13C-, and 15N-labeled L-serines

✍ Scribed by John Hanners; Rowena Gibson; Karen Velarde; Judy Hammer; Mark Alvarez; Janet Griego; Clifford J. Unkefer

Publisher
John Wiley and Sons
Year
1991
Tongue
French
Weight
460 KB
Volume
29
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

We have developed a stereospecific biosynthesis of ^13^C‐ and ^15^N‐labeled L‐serine which involves the serine‐type methylotroph Methylobacterium extorquens AM1. In this biosynthesis, C‐3 of serine is derived from methanol while C‐2, C‐1 and the α‐amino group are derived from glycine. By starting with the appropriate labeling precursor, we can produce any of the ^2^H, ^13^C and/or ^15^N isotopomers of L‐serine. Using a 5‐L culture, 40–50 millimoles of L‐serine are produced; L‐serine and glycine are recovered from the growth medium and then separated chromatographically.

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