Neoplastic transformation of fetal lamb kidney cells by bovine leukemia virus

✍ Scribed by Johng S. Rhim; Matthias Kraus; Paul Arnstein

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: French
• Weight: 539 KB
• Volume: 31
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910310620

✦ Synopsis

Abstract

Neoplastic transformation of fetal lamb kidney (FLK) cells by bovine leukemia virus (BLV) is reported. Morphological transformation was observed in FLK cells within a few weeks of BLV inoculation. BLV‐transformed FLK cells had the following properties generally associated with viral transformation: (1) altered morphology; (2) increased growth rate; (3) colony formation in soft agar medium; (4) formation of large cell aggregates and growth in this aggregate form above an agar base; and (5) tumorigenicity in nude mice. The transformed cells contained BLV‐specific p24 and gp51 antigens and were virus producers. BLV was detected in transformed cells by syncytial assay. The transformed cells yielded high‐titered viral reverse transcriptase activity and viral particles. The transformed cells contained marker chromosomes (M^1^, M<2>, M<3>, M^4^ and M^7^) and a large submetacentric chromosome, but control cells did not. Thus, we demonstrated the neoplastic transformation of FLK cells by BLV.