High resolution electrophoresis of extracts of a baby hamster kidney cell line before and after transformation by polyoma virus
✍ Scribed by Thomas Marshall; Professor Albert L. Latner
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 642 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
The polypeptide patterns obtained following denaturation and high resolution two‐dimensional electrophoresis of proteins from baby hamster kidney cells before and after transformation by Polyoma virus have been compared by silver staining incorporating methylamine. The polypeptides were resolved either by non‐equilibrium pH‐gradient electrophoresis or isoelectric focusing in the first dimension. Two different approaches to isoelectric focusing were employed in order to achieve satisfactory resolution over a wide range of isoelectric point. Both cell lines gave remarkably similar two‐dimensional patterns of spot distribution which allowed changes to be detected in the relative abundance of a number of polypeptides. None of the polypeptides detected were specific to either of the two cell lines.