Mutarotase effect on colorimetric determination of blood glucose with β-D-glucose oxidase
✍ Scribed by Ichitomo Miwa; Jun Okuda; Kazuo Maeua; Go Okuda
- Book ID
- 115819662
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 187 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A modification of Keston's glucose oxidase method for D-glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45 °, for 30 minutes. A clear stable color was developed by addition of sulfuric acid to a final concentration of 30 per cent.
In 1963, Keston and Brandt (1) first reported the determination of D-glucose anomers using a /3-n-glucose oxidase/peroxidase/chromogen system. Hill (2) developed an automated procedure using a similar system and measured deviations from equilibrium of anomers in the blood of dogs given epinephrine,
Glucosidase activities in plants and animals are normally measured by determining glucose production from suitable substrates. Two main methods are used for assaying micro quantities of glucose. In method a glucose formation is coupled with the reduction of NADP+ catalyzed by hexokinase and glucose-