Effect of sulfhydryl reagents on glucose determination by the glucose oxidase method

✍ Scribed by Diana M. Kilburn; Pamela M. Taylor

• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 198 KB
• Volume: 27
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(69)90069-4

✦ Synopsis

Glucosidase activities in plants and animals are normally measured by determining glucose production from suitable substrates. Two main methods are used for assaying micro quantities of glucose. In method a glucose formation is coupled with the reduction of NADP+ catalyzed by hexokinase and glucose-6-phosphate dehydrogenase. The NADPH is measured spectrophotometrically (1, 2). Method b involves the oxidation of P-glucose to gluconic acid by glucose oxidase, with the concurrent release of hydrogen peroxide, In the presence of peroxidase the peroxide oxidizes o-dianisidine to the corresponding imine, which is brown in neutral solution but pink in its protonnted form. This is measured colorimctrieally (3-6).