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Colorimetric estimation of d-glucose and 2-deoxy-d-glucose with glucose oxidase

✍ Scribed by R.B. McComb; W.D. Yushok

Publisher
Elsevier Science
Year
1958
Tongue
English
Weight
304 KB
Volume
265
Category
Article
ISSN
0016-0032

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✦ Synopsis

A modification of Keston's glucose oxidase method for D-glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45 °, for 30 minutes. A clear stable color was developed by addition of sulfuric acid to a final concentration of 30 per cent. This acidification served as a convenient means of stopping the reaction and increased the sensitivity of the method. The method has been expanded for use in the analysis of 2-deoxy-D-glucose.

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