Determination of intracellular 2-deoxy-d-glucose 6-phosphate and uridine diphosphate-2-deoxy-d-glucose
โ Scribed by P. Biely; S. Bauer
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 502 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0003-2697
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## Abstract A convenient method for the synthesis of ^18^Fโ2โdeoxyโ2โfluoroโDโglucose (4) and ^18^Fโ2โdeoxyโ2โfluoroโDโmannose (8) by the direct fluorination of 3,4,6โtriโ0โacetylโDโglucal with ^18^FโF~2~ is described. ^14^Cโ2โdeoxyโ2โfluoroโDโglucose has been synthesized from ^14^Cโ3,4,6โtriโ0โace
A modification of Keston's glucose oxidase method for D-glucose analysis is described. The glucose oxidase-peroxidase reaction was carried out in phosphate buffer, pH 5.9 at 45 ยฐ, for 30 minutes. A clear stable color was developed by addition of sulfuric acid to a final concentration of 30 per cent.
## Isfituto per la Chimica di Mofecofe o'i Wexsse Bi5iogic5 del C. N. R., viu T&am 6, E10072 Arm Fe f f ce, Napo fi (If a/y