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Molecular cloning and sequence analysis of theSchizosaccharomyces pombe ade10+ gene

✍ Scribed by Liedtke, Christian; Schmidt, Henning

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 170 KB
Volume: 14
Category: Article
ISSN: 0749-503X
DOI: 10.1002/(sici)1097-0061(1998100)14:14<1307::aid-yea308>3.0.co;2-4

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✦ Synopsis

We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5-phosphoribosyl-4carboxamide 5-aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupted open reading frame of 1755 nucleotides corresponding to 585 amino acid residues. The deduced amino acid sequence shows a high degree of similarity to the purH gene product of many species, including Saccharomyces cerevisiae, human, chicken and Escherichia coli. Moreover our data indicate that intrachromosomal recombination in Schiz. pombe is enhanced if the ade10 gene product is defective. The sequence has been submitted to the EMBL data library under Accession Number Y16419.

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