𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Molecular cloning and sequencing of a chitin synthase gene (CHS2) ofParacoccidioides brasiliensis

✍ Scribed by Niño-Vega, Gustavo A.; Buurman, Ed T.; Gooday, Graham W.; San-Blas, Gioconda; Gow, Neil A. R.

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
165 KB
Volume
14
Category
Article
ISSN
0749-503X

No coin nor oath required. For personal study only.

✦ Synopsis

The nucleotide sequence of a chitin synthase gene (CHS2) of the dimorphic fungal human pathogen Paracoccidioides brasiliensis has been determined. The deduced amino acid sequence of Chs2p consists of 1043 residues and is highly homologous to other class II fungal chitin synthases. Computational structural analyses suggest very high similarity to other fungal chitin synthases with a highly variable region at the cytosolic amino-terminal region which may be related to its possible zymogenic nature, and the putative catalytic region close to seven membrane-spanning regions at the carboxyl terminus.

📜 SIMILAR VOLUMES

Molecular cloning and characterization o
Molecular cloning and characterization of a glucan synthase gene from the human pathogenic fungusParacoccidioides brasiliensis
✍ Pereira, Maristela; Felipe, M. Sueli S.; Br�gido, Marcelo M.; Soares, C�lia M. A 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 420 KB 👁 2 views

1,3-b-D-glucan is a fungal cell wall polymer synthesized by the multi-subunit enzyme 1,3-b-D-glucan synthase. A subunit of this integral membrane protein was ®rst described as the product of the FKS1 gene from Saccharomyces cerevisiae using echinocandin mutants. Other FKS1 genes were also reported f

Molecular cloning and sequence analysis
Molecular cloning and sequence analysis ofZygosaccharomyces rouxii ADE2 gene encoding a phosphoribosyl-aminoimidazole carboxylase
✍ Sychrova, Hana; Braun, Valerie; Souciet, Jean-Luc 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB 👁 2 views

The nucleotide sequence of a 2•8 kb fragment containing the ADE2 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The gene was cloned from a Z. rouxii genomic DNA library by complementation of the Saccharomyces cerevisae ade2 mutant strain. The sequenced DNA fragment cont

Molecular cloning and transfection studi
Molecular cloning and transfection studies of M6b-2, a novel splice variant of a member of the PLP-DM20/M6 gene family
✍ Demetrius A. Vouyiouklis; Hauke Werner; Ian R. Griffiths; Gregor J. Stewart; Kla 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 296 KB 👁 1 views

The present study documents the nucleic acid and deduced amino acid sequence of M6b-2, a novel splice variant of the M6b gene, which belongs to the PLP-DM20/M6 gene family. M6b-2 differs from the previously published M6b by a novel 40-amino acid insertion which is characterised by a high proline con