Molecular cloning and sequence analysis ofZygosaccharomyces rouxii ADE2 gene encoding a phosphoribosyl-aminoimidazole carboxylase

✍ Scribed by Sychrova, Hana; Braun, Valerie; Souciet, Jean-Luc

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 233 KB
• Volume: 15
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(19990930)15:13<1399::aid-yea464>3.0.co;2-r

✦ Synopsis

The nucleotide sequence of a 2•8 kb fragment containing the ADE2 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The gene was cloned from a Z. rouxii genomic DNA library by complementation of the Saccharomyces cerevisae ade2 mutant strain. The sequenced DNA fragment contains a 1710 bp open reading frame predicting a protein of 570 amino acids. The deduced amino acid sequence shares a high degree of homology with Ade2p homologues in five other yeast species. The Gene Bank Accession No. of Z-ADE2 is AJ133372.