Nucleotide sequence of theSchizosaccharomyces pombe lys1+ Gene and Similarities of the lys1+ protein to peptide antibiotic synthetases
✍ Scribed by Bhattacherjee, Vasker; Bhattacharjee, Jnanendra K.
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 398 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0749-503X
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✦ Synopsis
The 4•2 kbp lys1 + gene of Schizosaccharomyces pombe encoding the large subunit of -aminoadipate reductase (EC1.2.1.31), an enzyme specific to lysine synthesis in higher fungi, was completely sequenced at the nucleotide level from pLYS1H. The S. pombe lys1 + gene product consists of 1415 amino acid residues and has a putative molecular weight of 155•8 kDa. The encoded protein converts -aminoadipic acid to -aminoadipate--semialdehyde by an ATP-mediated adenylation. Analysis of the sequence showed that the putative protein encoded by lys1 + shares strong homology with the peptide antibiotic synthetases which also use an adenylation step. The sequence data reported in this paper have been submitted to GenBank database (Washington DC, USA) under the Accession Number U15923. 1998