A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV ir
Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomyceteMicrobispora thermodiastatica
โ Scribed by A. Arai; S. Masuda; A. Matsuyama; S. Murakami; M. Nakajima
- Publisher
- Springer
- Year
- 1998
- Tongue
- English
- Weight
- 294 KB
- Volume
- 49
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Azospirillum brasilense isolated from the rhizosphere of different plants has the ability to excrete indole-3-acetic acid (IAA) into the culture media. Cosmid p0.2, isolated from an A. brasilense Sp245 genome library in pLAFR1, complements the Tn5-induced mutant SpM7918 of A. brasilense Sp6 which ex
The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has
The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM10