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Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli

✍ Scribed by Kano, Yasunobu ;Yoshino, Setsuo ;Wada, Morimasa ;Yokoyama, Kazushige ;Nobuhara, Masahiro ;Imamoto, Fumio

Publisher: Springer
Year: 1985
Tongue: English
Weight: 312 KB
Volume: 201
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00425687

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✦ Synopsis

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.

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