✦ LIBER ✦

The Vitreoscilla hemoglobin gene: Molecular cloning, nucleotide sequence and genetic expression in Escherichia coli

✍ Scribed by Khosla, Chaitan ;Bailey, James E.

Publisher: Springer
Year: 1988
Tongue: English
Weight: 530 KB
Volume: 214
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00340195

No coin nor oath required. For personal study only.

✦ Synopsis

Vitreoscilla hemoglobin is involved in oxygen metabolism of this bacterium, possibly in an unusual role for a microbe. We have isolated the Vitreoscilla hemoglobin structural gene from a pUC19 genomic library using mixed oligodeoxy-nucleotide probes based on the reported amino acid sequence of the protein. The gene is expressed in Escherichia coli from its natural promoter as a major cellular protein. The nucleotide sequence, which is in complete agreement with the known amino acid sequence of the protein, suggests the existence of promoter and ribosome binding sites with a high degree of homology to consensus E. coli upstream sequences. In the case of at least some amino acids, a codon usage bias can be detected which is different from the biased codon usage pattern in E. coli. The downstream sequence exhibits homology with the 3' end sequences of several plant leghemoglobin genes. E. coli cells expressing the gene contain greater than fivefold more heme than controls.

📜 SIMILAR VOLUMES

Molecular cloning and nucleotide sequenc

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli

✍ Kano, Yasunobu ;Yoshino, Setsuo ;Wada, Morimasa ;Yokoyama, Kazushige ;Nobuhara, 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 312 KB

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has

Genetic analysis and molecular cloning o

Genetic analysis and molecular cloning of the Escherichia coli ruv gene

✍ Shurvinton, Claire E. ;Lloyd, Robert G. ;Benson, Fiona E. ;Attfield, Paul V. 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 804 KB

The genetic organisation of the ruv gene, a component of the SOS system for DNA repair and recombination in Eseheriehia toll, was investigated. New point mutations as well as insertions and deletions were generated using transposon TnlO inserted in eda as a linked marker for site specific mutagenesi

The phosphoenolpyruvate carboxylase gene

The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression

✍ Eikmanns, Bernhard J. ;Follettie, Max T. ;Griot, Martin U. ;Sinskey, Anthony J. 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 935 KB

Cloning and expression of the pneumococc

Cloning and expression of the pneumococcal autolysin gene in Escherichia coli

✍ García, Ernesto ;García, José-Luís ;Ronda, Concepción ;Garcá, Pedro ;López, Rube 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 784 KB

Molecular cloning and expression of a β-

Molecular cloning and expression of a β-glucosidase gene from Ruminococcus albus in Escherichia coli

✍ Hiroyuki Honda; Takao Saito; Shinji Iijima; Takeshi Kobayashi 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 613 KB

Cloning and nucleotide sequence of the S

Cloning and nucleotide sequence of the Salmonella typhimurium LT2 metF gene and its homology with the corresponding sequence of Escherichia coli

✍ Stauffer, George V. ;Stauffer, Lorraine T. 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 684 KB