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Cloning and nucleotide sequence of the Salmonella typhimurium pepM gene

✍ Scribed by Rao Movva, N. ;Semon, Dominique ;Meyer, Christina ;Kawashima, Eric ;Wingfield, Paul ;Miller, Judith L. ;Miller, Charles G.

Publisher: Springer
Year: 1990
Tongue: English
Weight: 428 KB
Volume: 223
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00265075

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✦ Synopsis

The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM100, contained a single base change in the likely--35 region of the pepM promoter that increased its homology to the consensus promoter sequence. A region downstream from the pepM coding sequence contained extensive inverted repeats and was homologous to sequences found elsewhere in both Salmonella and other bacterial species.

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