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Molecular cloning and nucleotide sequence of the Rhizobium phaseoli recA gene

✍ Scribed by Michiels, Jan ;Vande Broek, Ann ;Vanderleyden, Jos

Publisher: Springer
Year: 1991
Tongue: English
Weight: 522 KB
Volume: 228
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00260644

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✦ Synopsis

A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV irradiation and the DNA alkylating agent methyl methanesulphonate (MMS). The R. phaseoli recA gene also promoted homologous recombination in E. coli. The cloned recA gene was only weakly inducible in E. coli recA strains by DNA damaging agents. The DNA sequence of the R. phaseoli recA gene was determined and compared with published recA sequences. No LexA-binding site was detected in the R. phaseoli recA upstream region.

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