## Abstract The refolding of recombinant protein from inclusion bodies expressed in __Escherichia coli__ can present a process bottleneck. Yields at industrially relevant concentrations are restricted by aggregation of protein upon dilution of the denatured form. This article studies the effect of
Modeling and Simulation of Fed-Batch Protein Refolding Process
β Scribed by Xiao-Yan Dong; Guang-Quan Shi; Wei Li; Yan Sun
- Book ID
- 109387201
- Publisher
- American Institute of Chemical Engineers
- Year
- 2004
- Tongue
- English
- Weight
- 108 KB
- Volume
- 20
- Category
- Article
- ISSN
- 8756-7938
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π SIMILAR VOLUMES
in the column enter. EMP permits to introduce the flow by a square-wave disturbance, avoiding back mixing close to plug flow. The chosen pulsation frequencies were determined in a previous study. Residence time distribution (RTD) analysis was calculated by step introducing a tracer into the column
## Abstract This article proposes a feeding strategy based on a kinetic model to enhance hairy roots growth. A new approach for modeling hairy root growth is used, considering that there is no nutrient limitation thanks to an appropriate feeding, and the intracellular pools are supposed to be alway