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Optimization of fed-batch refolding in downstream process of recombinant interferon β1b

✍ Scribed by A. Fazeli; S.A. Shojaossadati; M.R. Fazeli; H. Ilka

Publisher
Elsevier
Year
2009
Tongue
English
Weight
70 KB
Volume
25
Category
Article
ISSN
1871-6784

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✦ Synopsis

in the column enter. EMP permits to introduce the flow by a square-wave disturbance, avoiding back mixing close to plug flow.

The chosen pulsation frequencies were determined in a previous study. Residence time distribution (RTD) analysis was calculated by step introducing a tracer into the column for each pulsation and without pulsation. RTD analyses were fitted to axial dispersion model.

In this work, the mixing degree has been studied for different variables such as the settled bed height, the axial flow rate and pulsation frequencies. Bovine serum albumin protein was used in adsorption process to determine the best adsorption conditions.

The ␤-phycoerythrin adsorption in an expanded bed pulsing column was carried out in the best fluidodynamic and adsorption conditions which depend on the settled bed height, the axial flow rate and the pulsation frequencies.

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