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Microtiter assay for acetylcholinesterase

✍ Scribed by B.P. Doctor; L. Toker; E. Roth; I. Silman

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
348 KB
Volume
166
Category
Article
ISSN
0003-2697

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✦ Synopsis

A microtiter plate adaptation of the classical Ellman colorimetric procedure for measurement of acetylcholinesterase activity is described. This method permits use of an enzyme-linked immunosorbent assay plate reader for rapid analysis of multiple samples and is particularly suitable for analysis of acetylcholinesterase activity on sucrose gradients. The novel procedure is rapid and sensitive and does not require use of radioactive material.

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