𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Microdetermination of Proteins with the 1,10-Phenanthroline–H2O2–Cetyltrimethylammonium Bromide–Cu(II) Chemiluminescence System

✍ Scribed by Li Zheng Ping; Li Ke An; Tong Shen Yang

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
48 KB
Volume
60
Category
Article
ISSN
0026-265X

No coin nor oath required. For personal study only.

✦ Synopsis

Proteins can enhance the chemiluminescence (CL) intensity of the 1,10-phenanthroline-H 2 O 2cetyltrimethylammonium bromide (CTMAB)-Cu(II) system because unsaturated complexes of Cu(II) with protein have a much stronger catalytic effect on the CL reaction than does Cu(II). On this basis, a new flow injection analysis method for detection of some proteins was established. The method gives linear responses over two orders of magnitude and detection limits at the 0.02-0.05 g ml Ϫ1 level for bovine serum albumin, human serum albumin, ␥-globulin, and egg albumin. The method was used for determination of proteins in human serum with satisfactory results.

📜 SIMILAR VOLUMES

Synthesis and Crystal Structure of a Nov
Synthesis and Crystal Structure of a Novel Dinuclear Copper(II) Complex, [Cu2(phen)2(H2O)2(OH)2](HCO3)2 · 6 H2O with phen = 1,10-phenanthroline
✍ Yue-Qing Zheng; Jie Sun; Jian-Li Lin 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 German ⚖ 91 KB 👁 2 views

Dark blue plate-like crystals of [Cu 2 (phen) 2 (H 2 O) 2 (OH) 2 ](HCO 3 ) 2 ´6 H 2 O were obtained from a CH 3 OH±H 2 O solution containing CuCl 2 , 1,10-phenanthroline (phen), sebacic acid and Na 2 CO 3 . The crystal structure (triclinic, P 1 (no. 2), a = 8.118(1), b = 9.624(1), c = 10.536(1) A Ê

Study on Catalytic Effect of Copper(II)
Study on Catalytic Effect of Copper(II) Complex on 1,10-Phenanthroline-H2O2-CTMAB Chemiluminescence Reaction and Its Analytical Applications
✍ Z.P. Li; L.T. Dai; Z.J. Zhang 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 318 KB

Through our flow injection analysis experiments, we discovered that an unsaturated complex of \(\mathrm{Cu}\) (II) and organic ligand had a strong catalytic effect on 1.10 -phenanthroline- \(\mathrm{H}_{2} \mathrm{O}_{2}-\mathrm{CTMAB}\) chemiluminescence reaction. Based on this mechanism, the new f

A Novel Dinuclear Copper(II) Complex Mol
A Novel Dinuclear Copper(II) Complex Molecule: Synthesis and Crystal Structure of Cu2(phen)2(H2O)2(C5H6O4)2 with phen = 1,10-phenanthroline
✍ Yue-Qing Zheng; Jie Sun; Jian-Li Lin 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 German ⚖ 112 KB 👁 2 views

Blue crystals of Cu 2 (phen) 2 (H 2 O) 2 (C 5 H 6 O 4 ) 2 were obtained from a CH 3 OH±H 2 O solution containing CuCl 2 , 1,10-phenanthroline (phen), glutaric acid and Na 2 CO 3 . The crystal structure (monoclinic, P2 1 /c (no. 14), a = 10.271(1), b = 10.595(1), c = 15.585(1) A Ê , b = 107.105(3)°,

On the oxidation of saturated hydrocarbo
On the oxidation of saturated hydrocarbons with H2O2 in the presence of iron(II)-picolinate or iron(II)−1,10 phenanthroline 2-carboxylate.
✍ G. Balavoine; D.H.R. Barton; J. Boivin; A. Gref 📂 Article 📅 1990 🏛 Elsevier Science 🌐 French ⚖ 261 KB

A new catalytic system for highly selective oxidation of saturated hydrocarbons into ketones is described. This system is based on the combination offerrous picolinate (or l,lOphenanthroline 2-carboqylate) and H202 in pyridine sohuion.Under these conditions cyclohexane gave mabdy cyclohmanone (90% ,