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Microdetermination of neutral and amino sugars found in glycoproteins

✍ Scribed by Jon Barr; Philip Nordin

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
992 KB
Volume
108
Category
Article
ISSN
0003-2697

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✦ Synopsis

A liquid chromatographic method to analyze monosaccharides normally found in glycoprotein and glycopeptide hydrolysates is described. Monosaccharides are chromatographed as free sugars and as tritium-labeled alditols on an anion-exchange column in 0.5 M borate buffer, pH 8.6. Since the order of elution for aldoses and alditols is different, complete resolution of glucosamine, galactosamine, rhamnose, mannose, fucose, arabinose, galactose, xylose, and glucose can be obtained. Chromatography is complete in less than 1 h and the column does not require regeneration. The method was applied to the analysis of human immunoglobulin, human transfenin, and a glycopeptide from Acer pseudop/atanus cell cultures. The presence of amino acids in the hydrolysates does not interfere with analysis. * Contribution No. 80-170-J, Kansas Agricultural Experiment Station,

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