The rapid, quantitative determination of neutral sugars (as aldononitrile acetates) and amino sugars (as O-methyloxime acetates) in glycoproteins by gas-liquid chromatography
✍ Scribed by Thomas P. Mawhinney; Milton S. Feather; Giulio J. Barbero; J.Ricardo Martinez
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 452 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Methyloximes have been prepared from 2-amino-2-deoxy-D-glucose, -D-mannOSe, and -o-galactose. The acetates of these derivatives yield stable compounds which are readily separated quantitatively by gas-liquid chromatography on a number of polar phases. The above compounds along with the aldononitrile acetates of neutral sugars can be easily separated from one another on a single column in one chromatographic run. The procedures developed were tested on a number of glycoproteins of known composition as reported by other workers utilizing more classical methodologies, resulting in excellent agreement in terms of sugar composition. An improved method is also described for converting neutral sugars to oximes which can be either converted to the trimethylsilyl derivatives or, upon acetylation, derivatized to aldononitrile acetates. ' Issued as Journal Paper No. 8376 of the Missouri Agricultural Experiment Station.