Methyloximes have been prepared from 2-amino-2-deoxy-D-glucose, -D-mannOSe, and -o-galactose. The acetates of these derivatives yield stable compounds which are readily separated quantitatively by gas-liquid chromatography on a number of polar phases. The above compounds along with the aldononitrile
Gas chromatography of neutral and amino sugars in glycoproteins
β Scribed by William Niedermeier
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 618 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0003-2697
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A liquid chromatographic method to analyze monosaccharides normally found in glycoprotein and glycopeptide hydrolysates is described. Monosaccharides are chromatographed as free sugars and as tritium-labeled alditols on an anion-exchange column in 0.5 M borate buffer, pH 8.6. Since the order of elut
A rapid and sensitive procedure for the analysis of neutral and amino sugars is presented. Neutral sugars are separated after conversion to the corresponding glycamines, while the amino sugars are analyzed without modification, using an automatic amino acid analyzer and fluorometric detection. The m