A double beam scanning spectrodensitometer is used for the determination of DHEA (dehydroepiandrosterone = '3@hydroxyandrost-5-en-I7-one), :and?osterone (3p-hydroxy-ga-androstan-r7-one) and etiocholanolone (go-hydroxy-$3~andr&tanir$one) from urine. After ether solvolysis, purification and reaction w
Microdetermination of ketosteroids as 2,4-dinitrophenylhydrazones
β Scribed by Mazzella, Peter P. ;Ma, T. S.
- Publisher
- Springer-Verlag
- Year
- 1968
- Weight
- 164 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0344-838X
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π SIMILAR VOLUMES
Thin-layer chromatography of keto acid 2,4-dinitrophenylhydrazones was carried out on plate coated with silica gel and sodium bicarbonate. A clear separation was obtained between most of the various keto acid hydrazones. The hydrazone was eluted from the plate with methanol containing 1% concentrate
## We dedicate this work to a distinguished organic chemist, ΓakΔ±l ERK, who died in the summer of 2005. The o-dihydroxy-(2a-b), dimethoxy (3a-b), ethylendioxy-(4a-b) and macrocyclic polyethers (4c-i) are the initial compounds while the 2,4-dinitrophenylhydrazone compounds (5a-d, 6a-i) represent ne