✦ LIBER ✦

Microcomputer-automated reactor for synthesis of 13C-labeled Monosaccharides

✍ Scribed by Ulick Stafford; Anthony S. Serianni; Arvind Varma

Publisher: American Institute of Chemical Engineers
Year: 1990
Tongue: English
Weight: 694 KB
Volume: 36
Category: Article
ISSN: 0001-1541
DOI: 10.1002/aic.690361206

No coin nor oath required. For personal study only.

✦ Synopsis

For less labor-intensive, larger-scale, and more efficient production of D-[ 1-13C] glucose and other labeled monosaccharides using the cyanohydrin reduction reaction, an automated reactor was designed, constructed, and tested. A suitable vessel and all ancillary and control equipment were fabricated, or otherwise obtained, and the apparatus was controlled with a microcomputer program written in BASIC. Running without manual intervention, the yields for the condensation reaction of D-arabinose with KCN and the recovery of the excess cyanide were high (>go%), and the resultant aldononitriles were reduced to product aldoses with little reduction of the intermediate imines to amines. The reduction rate could be improved by controlling pH between 2-4 and by agitation speeds >250 rpm, while no improvement resulted from the use of high hydrogen pressures [>I29 kPa (4 psig)]. Reduction reactions conducted in the presence of formic acid (to regulate pH) suggest that formic acid acted as a coreducing agent.

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