Pathways for the synthesis of sorbitol from 13C-labeled hexoses, pentose, and glycerol in renal papillary tissue
✍ Scribed by A. W. H. Jans; R.-W. Grunewald; R. K. H. Kinne
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 226 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Suspensions of rabbit renal papillary tissue were incubated with ~-[ 6 -'3C]glucose, D-[ 1 -I3C] fructose, D-[ 1 -"C] ribose, and [ 2-I3C] glycerol. The perchloric acid extracts of the above incubations were investigated with I3C NMR spectroscopy. All I3C-labeled substrates give rise to 13C-labeled D-sorbitol. ~-[ 6 -~~C ] G l u c o s e and D[ 1-13C]fructose are converted directly into D-sorbitol via the aldose reductase and sorbitol dehydrogenase pathway, respectively, whereas D-[ 1-I3C] ribose and [ 2-I3C J glycerol give rise to labeling of the D-glyceraldehyde pool which on its turn causes a labeling of D-sorbitol. Label exchanges observed from incubations with glycerol and D-ribose indicate that the pentose shunt plays a role in this synthesis of D-sorbitol.