Methods for assay of trimethoprim and sulphadiazine in broiler tissues using liquid chromatography

✍ Scribed by M. Dagorn; J.M. Delmas

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 402 KB
• Volume: 285
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80075-8

✦ Synopsis

A process for the extraction of sulphadiazine (SDZ) and trimethoprim (TMP) from broiler fortified tissues (plasma, lung, liver, kidney, fat, muscle, skin) was developed and a liquid chromatographic (LC) method for their determination is described. After extraction in acidic medium (pH 6.8) with ethyl acetate or dichloromethane, the extracts were purified with hexane-chloroform in an acidic buffer. Reversed-phase LC separation with gradient elution (phosphate buffer-acetonitrile) was applied. The values obtained for the quantification limit for both drugs are 0.108 pg ml-' for plasma, 0.100 pg g-' for lung and 0.050 pg g -' for the other tissues. The detection limits range from 0.010 to 0.015 pg g-l or ml-' and from 0.020 to 0.025 pg g-' or ml-' for SDZ and TMP, respectively. Recoveries, established by comparison of peak areas obtained with standards and those with fortified tissue, ranged between 85.7 and 67.2%, depending on the tissue.