Assay of trimethoprim, sulfamethoxazole and its N4-acetyl metabolite in biological fluids by high-pressure liquid chromatography

✍ Scribed by Ascalone, V.

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 299 KB
• Volume: 3
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240030508

✦ Synopsis

Abstract

A reversed‐phase HPLC assay method was developed to determine levels of the drugs trimethoprim, sulfamethoxazole and the N~4~‐acetyl metabolite of the latter in human blood plasma and urine. The organic substances are extracted with ethyl acetate by a single extraction at pH 6.8. The method is rapid, accurate, sensitive and precise and well suited to routine determination of these drugs on patients during clinical pharmacokinetic investigations.