Method for the Determination of Vitamin K1(20)in Human Plasma by Stable Isotope Dilution/Gas Chromatography/Mass Spectrometry

✍ Scribed by Fauler, G.; Leis, H. J.; Schalamon, J.; Muntean, W.; Gleispach, H.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 468 KB
• Volume: 31
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199606)31:6<655::aid-jms339>3.0.co;2-t

✦ Synopsis

A method for the quantitative determination of vitamin Klo,, (VK), an essential cofactor in the carboxylation of clotting factors, is presented. The assay is based on gas chromatography/electron impact mass/spectrometry. The preparation of deuterium-labelled vitamin I(,(,,, for use as an internal standard is described. The method involves extraction of VK from human plasma and its derivatization to the heptatluorobutyryl ester after reduction of one carbonyl group with zinc. The detection limit was found to be 1.0 pg and the limit of quantitation 2.0 pg ml-' plasma. This permits the measurement of vitamin K,(,,, even in small quantities of plasma, which is highly desirable in investigations dealing with clotting abnormalities in neonates and infants.