Metal-binding sites at the active site of restriction endonuclease BamHI can conform to a one-ion mechanism

✍ Scribed by Mones, Letif; Simon, István; Fuxreiter, Monika

• Book ID: 125486677
• Publisher: Walter de Gruyter GmbH & Co. KG
• Year: 2007
• Tongue: English
• Weight: 142 KB
• Volume: 388
• Category: Article
• ISSN: 1431-6730
• DOI: 10.1515/BC.2007.009

✦ Synopsis

The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca 2q and Mn 2q ions observed in the pre-and post-reactive complexes of BamHI conform to the classical two-metal ion choreography. We probed the Mg 2q cofactor positions at the active site of BamHI by molecular dynamics simulations with one and two metal ions present and identified several catalytically relevant sites. These can mark the pathway of a single ion during catalysis, suggesting its critical role, while a regulatory function is proposed for a possible second ion.