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Metal-binding sites at the active site of restriction endonuclease BamHI can conform to a one-ion mechanism

✍ Scribed by Mones, Letif; Simon, István; Fuxreiter, Monika

Book ID
125486676
Publisher
Walter de Gruyter GmbH & Co. KG
Year
2007
Tongue
English
Weight
142 KB
Volume
388
Category
Article
ISSN
1431-6730

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Metal-binding sites at the active site o
Metal-binding sites at the active site of restriction endonuclease BamHI can conform to a one-ion mechanism
✍ Mones, Letif; Simon, István; Fuxreiter, Monika 📂 Article 📅 2007 🏛 Walter de Gruyter GmbH & Co. KG 🌐 English ⚖ 142 KB

The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca 2q and Mn 2q ions observed in the pre-and post-reactive complexes of BamHI conform to the classical two-metal ion choreography. We probed the Mg 2q