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Divalent Metal Ions at the Active Sites of the EcoRV and EcoRI Restriction Endonucleases

✍ Scribed by Vipond, I. Barry; Baldwin, Geoffrey S.; Halford, Stephen E.


Book ID
120401092
Publisher
American Chemical Society
Year
1995
Tongue
English
Weight
980 KB
Volume
34
Category
Article
ISSN
0006-2960

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The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca 2q and Mn 2q ions observed in the pre-and post-reactive complexes of BamHI conform to the classical two-metal ion choreography. We probed the Mg 2q